产品信息

蛋白质全称	肝细胞核因子3α
别名	forkhead box A1, Forkhead box protein A1, FOXA1, HNF 3 alpha, HNF-3A, HNF3A, TCF-3A, TCF3A, Transcription factor 3A
Uniprot ID	P35582
免疫原	小鼠FOXA1重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	49 kDa / 49 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 1000-1: 2000	T-47D细胞, PC-3细胞, HepG2细胞

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背景

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

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图像

WB检测FOXA1蛋白(货号 GB113299). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 2000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.