Anti-c-Jun Rabbit pAb
- 50 μL
产品信息
蛋白质全称 | 转录因子AP-1 |
别名 | Activator protein 1, AP1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, JUN, Transcription factor AP-1, Proto oncogene cJun, AP-1 transcription factor subunit |
Uniprot ID | P05412 |
免疫原 | KLH偶联的人c-Jun合成肽 |
抗体亚型 | IgG |
预测分子量./观测分子量. | 36 kDa / 43 kDa |
纯化方式 | 亲和纯化 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 人, 小鼠, 大鼠 | 1: 500-1: 1000 | HeLa细胞, HepG2细胞, 293T细胞 |
背景
c-Jun is a protein that in humans is encoded by the JUN gene. c-Jun in combination with c-Fos, forms the AP-1early response transcription factor. It was first identified as the Fos-binding protein p39 and only later rediscovered as the product of the c-jun gene. It is activated through double phosphorylation by the JNK pathway but has also a phosphorylation-independent function. c-jun knockout is lethal, but transgenic animalswith a mutated c-jun that cannot be phosphorylated (termed c-junAA) can survive.
图像
| WB检测c-Jun蛋白(货号 GB111604). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(G2025)稀释本产品.
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