产品信息

蛋白质全称	异黏蛋白
别名	3D3/LYRIC, Lysine-rich CEACAM1 co-isolated protein, Metadherin, Metastasis adhesion protein, Mtdh, Lyric
Uniprot ID	Q80WJ7
免疫原	KLH偶联的小鼠LYRIC合成肽
抗体亚型	IgG
预测分子量./观测分子量.	64 kDa / 75 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 500-1: 1000	心, 脑

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背景

LYRIC, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is over expressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its over expression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression.

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图像

WB检测LYRIC/AEG1蛋白(货号 GB111005). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.