DESCRIPTION Gene Name Synonym VCAM1, CD106, INCAM-100, V-CAM 1, VCAM-1 Species Human A ccession P19320 Source HEK293 cells SPECIFICATIONS Amino acid sequence Phe25-Glu698 is expressed with a 10×His tag at the N terminus Molecular Weight 78.8 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU·mg -1 Formulation Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND Vascular cell adhesion protein 1 (VCAM1) is also known as CD106, INCAM-100 and L1CAM, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α4ß7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes. Data SDS-PAGE Bioactivity Recombinant Human VCAM1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 65-110 kDa. Immobilized Human VCAM1, His Tag at 5 μg/mL (100 μL/well) can bind Anti-VCAM1 with a linear range of 0.009-0.078 ng/mL. 仅供科研用途，不可用于临床诊断！