DESCRIPTION Gene Name Synonym CD74, DHLAG, HLADG, II, Ia-GAMMA, p33, CLIP, INVG34 Species Human Accession P04233 Source HEK293 cells SPECIFICATIONS Amino acid sequence Gln73-Met296 is expressed with a 10×His tag at the N terminus Molecular Weight 25.3 kDa（The protein migrates as 20~40 kDa under reducing condition (SDS-PAGE) due to glycosylation） Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU mg -1 Formulation Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND CD74 (MHC class II invariant chain, Ii) is a non-polymorphic type II transmembrane glycoprotein. CD74 has a diversity of biological functions in physiological and pathological situations. CD74 is expressed in a number of cell types independently of MHC class II. CD74 is a cell membrane high-affinity receptor for macrophage migration inhibitory factor (MIF), D-dopachrome tautomerase (D-DT/MIF-2) and bacterial proteins. CD74 regulates T-cell and B-cell developments, dendritic cell (DC) motility, macrophage inflammation, and thymic selection. CD74 plays important roles in many inflammatory diseases, such as liver fibrosis, type I diabetes, systemic lupus erythematosus, and Alzheimer disease. CD74 may be a therapeutic target to treat the relevant immune disorders in the near future. Data SDS-PAGE Bioactivity Active Recombinant Human CD74 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 35-40 kDa. Immobilized Human CD74, His Tag at 5 μg/mL (100 μL/well) can bind Anti-CD74 with a linear range of 0.02-0.15 μg/mL. 仅供科研用途，不可用于临床诊断！