DESCRIPTION Gene Name Synonym CD86, B7-2, B70, CD28LG2, LAB72, MGC34413 Species Human Accession P42081 Source HEK293 cells SPECIFICATIONS Amino acid sequence Ala24-Pro247 is expressed with a 10×His tag at the N terminus Molecular Weight 25.6 kDa（The protein migrates as 40~60 kDa under reducing condition (SDS-PAGE) due to glycosylation） Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU mg -1 Formulation Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND CD86, also known as B-lymphocyte activation antigen B7-2，is a member of the cell surface immunoglobulin superfamily. CD86 provides a stimulatory signal to B cell function. CD86 has also been shown to be important in initiation of IL-4 responses. The B7 (CD80, CD86) molecules on APC and their ligands on T cells (CD28, CTLA4 [CD152]) provide crucial costimulation signals for the regulation of effective immune responses. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses. Data SDS-PAGE Bioactivity Testing in progress Recombinant Human CD86 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 42-65 kDa. 仅供科研用途，不可用于临床诊断！