产品信息 蛋白质全称 赖氨酸特异性去甲基化酶5A 别名 Histone demethylase JARID1A, Jumonji/ARID domain-containing protein 1A, Kdm5a, Rbp2, RBBP-2, Jarid1a, Retinoblastoma-binding protein 2 Uniprot ID Q3UXZ9 免疫原 小鼠KDM5A重组蛋白 抗体亚型 IgG 预测分子量./观测分子量. 192 kDa / 192 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 人, 小鼠, 大鼠 1: 500-1: 1000 子宫, 肝 背景 The protein encoded by this gene is a ubiquitously expressed nuclear protein. It binds directly, with several other proteins to retinoblastoma protein which regulates cell proliferation. This protein also interacts with rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis. Rhombotin-2 is thought to either directly affect the activity of the encoded protein or may indirectly modulate the functions of the retinoblastoma protein by binding to this protein. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. 图像 WB检测KDM5A/Jarid1A/RBBP2蛋白(货号 GB111087). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.