产品信息 蛋白质全称 赖氨酸特异性去甲基酶6B 别名 Histone demethylase JMJD3, JmjC domain containing protein 3, Jumonji D3, Jumonji domain containing 3, Kdm6b, KIAA0346, Lysine demethylase 6B, Lysine K specific demethylase 6B, Lysine specific demethylase 6B Uniprot ID Q5NCY0 免疫原 小鼠KDM6B / JMJD3重组蛋白 抗体亚型 IgG 预测分子量./观测分子量. 176 kDa / 176 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 小鼠, 大鼠 1: 300-1: 600 脑 背景 Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. 图像 WB检测KDM6B/JMJD3蛋白(货号 GB114024). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.