Product Information Description LC3A/B rabbit polyclonal Protein full name Microtubule-associated proteins 1A/1B light chain 3 Synonyms LC3, LC3A, LC3A/B, LC3B, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A, MAP1LC3A/B, MAP1LC3B Immunogen LC3 fusion protein Ag6144 Isotype IgG Purity Affinity purification Subcellular location Cytoskeleton Uniprot ID Q9GZQ8 , Q9H492 , Q62625 , Q6XVN8 , Q9CQV6 , Q91VR7 Applications Applications Species Dilution Positive Tissue IHC Human, Mouse, Rat 1:300-1:1200 brain, lung Background LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I and LC3-II. Images Storage Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. NOTE: 1.This product is intended for research only. 2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （ G2025 ）.