Product Information Description ATG5 Mouse polyclonal Protein full name ATG5 autophagy related 5 homolog (S. cerevisiae) Synonyms APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5 Immunogen Recombinant protein corresponding to Mouse ATG5OIKJ Isotype IgG2a Purity Affinity purification Subcellular location Centrosome Predicted MW. 32 kDa Observed MW. 50-55/40-45 kDa Uniprot ID Q9H1Y0 Applications Applications Species Dilution Positive Tissue WB Human, Mouse, Rat 1:1000-1:6000 HEK-293, HeLa, Jurkat, Raji, HSC-T6, NIH/3T3 Background ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. Images Storage Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. NOTE: 1.This product is intended for research only. 2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （ G2025 ）.