产品信息 蛋白质全称 Beclin-1自噬相关蛋白 别名 Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, BECN1, GT197, APG6, VPS30, Protein GT197, beclin1, beclin-1 Uniprot ID Q14457 免疫原 人Beclin 1重组蛋白 抗体亚型 IgG1, κ 克隆号 3D2H4 预测分子量./观测分子量. 52 kDa / 60 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 人, 小鼠 1: 500-1: 1000 HeLa细胞, 脑, 结肠 背景 Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl 2 binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. 图像 WB检测Beclin 1蛋白(货号 GB125741). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗小鼠IgG (H+L) (货号GB23301), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.