产品信息 蛋白质全称 微管相关蛋白1A/1B轻链3B 别名 LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1LC3B Uniprot ID Q9GZQ8,Q62625,Q9CQV6 免疫原 KLH偶联的小鼠LC3B合成肽 预测分子量./观测分子量. 15 kDa / 14/16 kDa 纯化方式 亲和纯化 偶联 辣根过氧化物酶 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 人, 小鼠, 大鼠 1: 500-1: 1000 NIH3T3细胞, HeLa细胞, C6细胞 孵化条件 封闭后, 将膜与HRP标记的抗LC3B 抗体(ZB113801-HRP)在室温（20-25℃）孵育3-4小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟. 背景 Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria. While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. 图像 WB检测LC3B蛋白(货号 ZB113801-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3% BSA（货号GC305010）的PBS溶液, 室温孵育30分钟. 抗体: HRP标记的抗LC3B蛋白抗体 1: 500稀释, 4℃ 孵育过夜. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色. NOTE: 1.This product is intended for research only. 2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （ G2025 ）.