Product Information Protein full name Interferon regulatory factor 4 Synonyms EXPAND1, HSPC211, MUM 1, MUM1, Protein expandere Immunogen Recombinant protein corresponding to Human MUM1 Uniprot ID Q15306 Isotype IgG2a,k Purity Affinity purification Subcellular location Nucleus Predicted MW. / Observed MW. 50 kDa / 50 kDa Applications IHC/IF Human, Mouse, Rat 1: 300-1: 900 colon, spleen Background Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity). Images IHC analysis of MUM1(GB12161). Sample: Human tonsil, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. IHC analysis of MUM1(GB12161). Sample: Human colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. IHC analysis of MUM1(GB12161). Sample: Human esophageal carcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. IHC analysis of MUM1(GB12161). Sample: Mouse colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. IHC analysis of MUM1(GB12161). Sample: Mouse spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. IHC analysis of MUM1(GB12161). Sample: Rat colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. IHC analysis of MUM1(GB12161). Sample: Rat spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 300, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. Storage Storage Store at -20 ℃ for one year....