产品信息 蛋白质全称 cAMP/cGMP 3',5'-环磷酸二酯酶10A 别名 Pde10a, 5''-cyclic phosphodiesterase 10A, cAMP and cAMP-inhibited cGMP 3'', FLJ11894, Phosphodiesterase 10A, Phosphodiesterase 10A1 Uniprot ID Q8CA95 免疫原 小鼠PDE10A重组蛋白 抗体亚型 IgG 预测分子量./观测分子量. 89 kDa / 75 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 小鼠, 大鼠 1: 500-1: 1000 脑, 睾丸 背景 The PDE10A plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides，eliminate cAMP- and cGMP-mediated intracellular signaling by hydrolyzing the cyclic nucleotide to the corresponding nucleoside 5-prime monophosphate. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. 图像 WB检测PDE10A蛋白(货号 GB111420). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.