产品信息 蛋白质全称 多功能ADE2蛋白 别名 ADE2, ADE2H1, AIR carboxylase, AIRC, PAICS, PAIS, SAICAR synthetase, Phosphoribosylaminoimidazole carboxylase, Multifunctional protein ADE2H1, synthetase Uniprot ID Q9DCL9 免疫原 小鼠PAICS重组蛋白 抗体亚型 IgG 预测分子量./观测分子量. 47 kDa / 47 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 人 1: 1000-1: 3000 HeLa细胞, HepG2细胞, Jurkat细胞 背景 PAICS is also named as ADE2(Multifunctional protein), AIRC, PAIS. It is an important bifunctional enzyme in de novo purine biosynthesis in vertebrate with both 5-aminoimidazole ribonucleotide carboxylase (AIRc) and 4-(N-succinylcarboxamide)-5-aminoimidazole ribonucleotide synthetase (SAICARs) activities. It becomes an attractive target for rational anticancer drug design, since rapidly dividing cancer cells rely heavily on the purine de novo pathway for synthesis of adenine and guanine, whereas normal cells favor the salvage pathway. 图像 WB检测PAICS/PAIS蛋白(货号 GB113869). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 3000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.