产品信息 蛋白质全称 钠氢交换调节辅因子NHE-RF2 别名 NHERF-2, NHE3 kinase A regulatory protein E3KARP, Octs2, SRY-interacting protein 1, SIP-1, TKA-1, Slc9a3r2, Nherf2 Uniprot ID Q9JHL1 免疫原 小鼠NHERF-2/SIP-1重组蛋白 抗体亚型 IgG 预测分子量./观测分子量. 37 kDa / 37 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 小鼠, 大鼠 1: 1000-1: 2000 脑, 肾, 结肠 背景 This gene encodes a member of the NHERF family of PDZ scaffolding proteins. These proteins mediate many cellular processes by binding to and regulating the membrane expression and protein-protein interactions of membrane receptors and transport proteins. The encoded protein plays a role in intestinal sodium absorption by regulating the activity of the sodium/hydrogen exchanger 3, and may also regulate the cystic fibrosis transmembrane regulator (CFTR) ion channel. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. 图像 WB检测NHERF-2/SIP-1蛋白(货号 GB112708). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.