
Anti-NAALADase Rabbit pAb
- 100 μL
产品信息
|
蛋白质全称 |
推定N-乙酰α-连接酸性二肽酶 |
|
别名 |
PSMAL, GIG26, FOLH1B |
|
Uniprot ID |
Q9HBA9 |
|
免疫原 |
KLH偶联的人FOLH1B合成肽 |
|
抗体亚型 |
IgG |
|
预测分子量./观测分子量. |
50 kDa / 55 kDa |
|
纯化方式 |
亲和纯化 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 人 | 1: 2000-1: 4000 | K562细胞, A549细胞, HepG2细胞 |
背景
Prostate cancer is the most frequently diagnosed cancer and the early detection of prostate cancer dramatically and efficiently reduces the observed mortality rate. Several proteins have been identified as specific markers of prostate cancer, and they may be useful as diagnostic indicators. PSA, prostate specific antigen, is the classical indicator for transformed prostate tissue; however, in addition to being upregulated in prostate cancer, PSA is also upregulated in non-malignant conditions, such as benign prostatic hyperplasia prostate. Conversely, STEAP (six-transmembrane epithelial antigen of the prostate), prostate carcinoma tumor antigen (PCTA-1) and prostate-specific membrane antigen (PSM) represent additional prostate-specific antigens that are overexpressed only in malignant tumors and therefore are more specific identifiers of malignancies.
图像
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WB检测NAALADase蛋白(货号 GB115129). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 2000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(G2025)稀释本产品.
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