产品信息 蛋白质全称 丝裂原活化蛋白激酶激酶激酶12 别名 Dual leucine zipper bearing kinase, Leucine-zipper protein kinase, DLK, ZPK, MAPK-upstream kinase, MUK, Mixed lineage kinase, Map3k12 Uniprot ID Q60700 免疫原 小鼠MAP3K12重组蛋白 抗体亚型 IgG 纯化方式 亲和纯化 亚细胞定位 细胞膜, 细胞质 应用 应用 物种 稀释 阳性样品 IHC/IF 免疫组织化学/免疫荧光 人 1: 200-1: 400 肾 背景 This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described. 图像 IHC检测MAP3K12蛋白(货号 GB113489). 样品: 人肾, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 200稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.