产品信息 蛋白质全称 错构瘤蛋白 别名 Tsc1, TSC1, LAM, TSC, tuberous sclerosis 1, TSC complex subunit 1, TSC-1 Uniprot ID Q9EP53 免疫原 小鼠TSC-1重组蛋白 抗体亚型 IgG 预测分子量./观测分子量. 128 kDa / 128 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 人 1: 500-1: 1000 PC3细胞, Jurkat细胞, 293T细胞 背景 Tuberous sclerosis 1 (TSC1), also known as hamartin, is a protein encoded by the TSC1 gene. TSC1 functions as a co-chaperone which inhibits the ATPase activity of the chaperone Hsp90 (heat shock protein-90) and decelerates its chaperone cycle. Tsc1 functions as a facilitator of Hsp90 in chaperoning the kinase and non-kinase clients including Tsc2, therefore preventing their ubiquitination and degradation in the proteasome. 图像 WB检测TSC-1蛋白(货号 GB11882). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.