产品信息 蛋白质全称 Rho 鸟嘌呤核苷酸交换因子 1 别名 Lbc's second cousin, Lymphoid blast crisis-like 2, Arhgef1, Lbcl2, Lsc, 115 kDa guanine nucleotide exchange factor, GEF1, Lsc homolog, p115RhoGEF, LBCL2LSC Uniprot ID Q92888 免疫原 KLH偶联的人p115-RhoGEF合成肽 抗体亚型 IgG 预测分子量./观测分子量. 103 kDa / 80 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 人 1: 1000-1: 2000 HeLa细胞, 293T细胞, A549细胞 背景 This protein seems to play a role in the regulation of RhoA GTPase by guanine nucleotide binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation. 图像 WB检测p115-RhoGEF蛋白(货号 GB112380). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.