产品信息 蛋白质全称 GTP酶ERas 别名 E-Ras, Embryonic stem cell-expressed Ras, Eras, ecat5, HRAS2, HRASP, Small GTPase protein E Ras, v Ha ras Harvey rat sarcoma viral oncogene homolog 2, ES cell expressed Ras Uniprot ID Q7TN89 免疫原 小鼠ERAS重组蛋白 抗体亚型 IgG 预测分子量./观测分子量. 24 kDa / 24 kDa 纯化方式 亲和纯化 应用 应用 物种 稀释 阳性样品 WB 免疫印迹 大鼠 1: 500-1: 1000 睾丸 背景 ERAS belongs to the small GTPase superfamily. Ras family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. The ERas gene is expressed in embryonic stem (ES) cells and promotes their in vitro proliferation and tumorigenicity. ERas-null ES cells maintain pluripotency but show significantly reduced growth and tumorigenicity, which are rescued by expression of ERas complementary DNA or by activated phosphatidylinositol-3-OH kinase. 图像 WB检测ERAS蛋白(货号 GB113047). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. 储存 储存条件 在-20℃下储存一年, 避免反复冻融. 储存缓冲液 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. 注意: 1.本产品仅供研究使用. 2.建议用一抗稀释液( G2025 )稀释本产品.