产品信息

蛋白质全称	DNA双链断裂修复蛋白rad21同源物
别名	hHR21, Nuclear matrix protein 1, NXP-1, SCC1 homolog, 64-kDa carboxy-terminal product, Rad21, HR21, KIAA0078, NXP1, SCC1
Uniprot ID	Q61550
免疫原	小鼠Rad21重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	72 kDa / 75 /130 kDa
纯化方式	亲和纯化
亚细胞定位	细胞核, 染色体, 着丝粒, 细胞质, 胞质溶胶

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	HeLa细胞, THP-1细胞, K562细胞
ICC/IF 免疫细胞化学/免疫荧光	人	1: 100-1: 400	HeLa细胞

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背景

RAD21, also named as Nuclear matrix protein 1, which belongs to the rad21 family. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

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图像

	WB检测Rad21蛋白(货号 GB111435). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.
	ICC检测Rad21蛋白(货号 GB111435)(红色). 样品: Hela细胞, 4%多聚甲醛 (货号G1101) 固定20分钟. 封闭: 3% BSA（货号GC305010）的PBS溶液, 室温孵育30分钟. 一抗: 1: 400稀释, 4℃ 孵育过夜. 二抗: Cy3标记山羊抗兔IgG (H+L) (货号GB21303), 1: 300稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.