Anti-CDK9 Rabbit pAb
- 50 μL
产品信息
蛋白质全称 | 细胞周期蛋白依赖性激酶9 |
别名 | Cell division protein kinase 9, Cdk9, C2k, CDC2L4, CTK1, PITALRE, TAK |
Uniprot ID | |
免疫原 | 小鼠Cdk9重组蛋白 |
抗体亚型 | IgG |
纯化方式 | 亲和纯化 |
亚细胞定位 | 细胞核, 细胞质 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| IHC/IF 免疫组织化学/免疫荧光 | 大鼠 | 1: 500-1: 1000 | 脑 |
背景
CDK9 (cyclin-dependent kinase 9) is a member of the cyclin-dependent protein kinase family. It is involved in the regulation of transcription. CDK9 is a member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex. It regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors. CDK9 promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis. The CDK9/cyclin-K complex also has kinase activity towards CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 causes cardic myocyte enlargement leading to cardiac hypertrophy and confers a predisposition to heart failure.
图像
| IF检测CDK9蛋白(货号 GB111389)(红色). 样品: 大鼠脑海马体, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: Tris-EDTA抗原修复液(pH 9.0) (G1203), 98℃, 20分钟. 封闭: 3% BSA（货号GC305010）的PBS溶液, 室温孵育30分钟. —抗: 1: 600稀释, 4℃ 孵育过夜. 二抗: Cy3标记山羊抗兔IgG (H+L) (货号GB21303), 1: 300稀释, 室温孵育1小时. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(G2025)稀释本产品.
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