产品信息

蛋白质全称	双特异性蛋白激酶TTK
别名	Phosphotyrosine picked threonine-protein kinase, PYT, TTK, MPS1, MPS1L1, ESK, TTK protein kinase
Uniprot ID	P35761
免疫原	KLH偶联的小鼠TTK合成磷酸肽
抗体亚型	IgG
预测分子量./观测分子量.	96 kDa / 100 kDa
纯化方式	亲和纯化

---

应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠	1: 300-1: 500	睾丸

---

背景

Mps1 family of kinases colocalizes with mitotic checkpoint proteins in kinetochores and/or spindle pole bodies/centrosomes. TTK has been shown to be a cell cycle-regulated kinase displaying maximum activity during M phase. Its kinase activity is required for proper chromosome segregation during mitosis through its involvements in microtubule-chromosome attachment error correction and the mitotic checkpoint.

---

图像

WB检测TTK/Mps1蛋白(货号 GB115460). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 300稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

---

储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

---

注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.