Anti-14-3-3 alpha+beta Rabbit pAb
- 50 μL
产品信息
蛋白质全称 | 14-3-3蛋白β/α亚型 |
别名 | YWHAB, GW128, HEL-S-1, HS1, KCIP-1, YWHAA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta |
Uniprot ID | Q9CQV8 |
免疫原 | KLH偶联的小鼠14-3-3 alpha + beta合成肽 |
抗体亚型 | IgG |
预测分子量./观测分子量. | 28 kDa / 28 kDa |
纯化方式 | 亲和纯化 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 小鼠, 大鼠 | 1: 500-1: 1000 | 肺, 脑 |
背景
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.
图像
| WB检测14-3-3 alpha + beta蛋白(货号 GB11593). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(G2025)稀释本产品.
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