产品信息

蛋白质全称	丝氨酸/苏氨酸蛋白激酶Chk2
别名	CHEK2, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1, Checkpoint kinase 2
Uniprot ID	Q9Z265
免疫原	小鼠Chk2重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	61 kDa / 61 kDa
纯化方式	亲和纯化

---

应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠	1: 500-1: 1000	肾, 结肠, 胸腺

---

背景

CHEK2 (Checkpoint kinase 2) is a tumor suppressor gene that encodes the protein CHK2, a serine-threonine kinase. CHK2 regulates cell division, and has the ability to prevent cells from dividing too rapidly or in an uncontrolled manner, So CHK2 is mainly involved in DNA repair, cell cycle arrest or apoptosis in response to DNA damage. When DNA undergoes a double-strand break, CHK2 is activated. Specifically, DNA damage-activated phosphatidylinositol kinase family protein (PIKK) ATM phosphorylates site Thr68 and activates CHK2.

---

图像

WB检测Chk2蛋白(货号 GB11574). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

---

储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

---

注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.