产品信息

蛋白质全称	细胞周期蛋白依赖性激酶6
别名	Serine/threonine-protein kinase PLSTIRE, CDK6, CDKN6, MCPH12, MGC59692, PLSTIRE, STQTL11, Crk2, Cell division protein kinase 6
Uniprot ID	Q00534
免疫原	KLH偶联的人CDK6合成肽
抗体亚型	IgG
预测分子量./观测分子量.	37 kDa / 37 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	Jurkat细胞, K562细胞, HEK293细胞

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背景

Cyclin-dependent kinase 6 (p40cdk6 also known as PLSTIRE) belongs to a family of proteins designated as cdks. They are critical regulators of cell cycle progression. The prototype member of this family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression. CDK6 is essential for cell proliferation within the dentate gyrus of the hippocampus and the cuventricular zone of the lateral ventricles. Mutations in the gene results in microcephaly 12, primary, autosomal recessive (MCPH12).

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图像

WB检测CDK6蛋白(货号 GB111606). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.