Recombinant Anti-GAPDH antibody (HRP Conjugated)
- 100 μL
产品信息
蛋白质全称 | 甘油醛-3-磷酸脱氢酶 |
别名 | GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase |
Uniprot ID | P16858 |
免疫原 | KLH偶联的小鼠GAPDH合成肽 |
预测分子量./观测分子量. | 36 kDa / 36 kDa |
纯化方式 | 亲和纯化 |
偶联 | 辣根过氧化物酶 |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 人, 小鼠, 大鼠, 猪, 鸡, 牛, 羊, 山羊, 猴, 兔, 小麦, 青蛙 | 1: 1000-1: 3000 | HeLa细胞, HepG2, 脑, etc. |
| 孵化条件 | 封闭后, 将膜与HRP标记的抗GAPDH抗体（ZB15004-HRP）在室温（20-25℃）孵育2-3小时或4℃过夜, 随后用TBST缓冲液洗涤3次,每次5分钟. | ||
背景
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport. In sperm, a testis-specific isoenzyme GAPDHS is expressed.
图像
| WB检测GAPDH蛋白(货号 ZB15004-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. 抗体: HRP标记的抗GAPDH蛋白抗体 1: 3000稀释, 4℃ 孵育过夜. |
| WB检测GAPDH蛋白(货号 ZB15004-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. 抗体: HRP标记的抗GAPDH蛋白抗体 1: 3000稀释, 4℃ 孵育过夜. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(G2025)稀释本产品.
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