产品信息

蛋白质全称	胞质肌动蛋白1
别名	ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin, β-Actin
Uniprot ID	P60710
预测分子量./观测分子量.	42 kDa / 42 kDa
纯化方式	亲和纯化
偶联	辣根过氧化物酶

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人, 小鼠, 大鼠, 猪, 鸡, 牛, 羊, 山羊, 猴, 兔, 小麦, 青蛙	1: 1000-1: 3000	HeLa细胞, HepG2, 脑, etc.
孵化条件	封闭后, 将膜与HRP标记的beta Actin抗体（ZB15001-HRP）在室温（20-25℃）孵育2-3小时或4℃过夜, 随后用TBST缓冲液洗涤3次, 每次5分钟.

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背景

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

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图像

WB检测beta Actin蛋白(货号 ZB15001-HRP). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. 抗体: HRP标记的抗beta Actin蛋白抗体 1: 3000稀释, 4℃ 孵育过夜.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.产品颜色为浅黄色.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.