产品信息

蛋白质全称	G蛋白激活内向整流钾通道4
别名	GIRK-4, Cardiac inward rectifier, Heart KATP channel, CIR, KATP-1, Heart KATP channel, Kcnj5, Girk4, Inward rectifier K(+) channel Kir3.4
Uniprot ID	P48545
免疫原	KLH偶联的小鼠KCNJ5合成肽
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	膜

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应用

应用	物种	稀释	阳性样品
IHC/IF 免疫组织化学/免疫荧光	大鼠	1: 400-1: 800	心

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背景

This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.

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图像

IHC检测KCNJ5蛋白(货号 GB113854). 样品: 大鼠心, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 800稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.