产品信息

蛋白质全称	溶质载体家族22成员5
别名	High-affinity sodium-dependent carnitine cotransporter, Organic cation/carnitine transporter 2, Slc22a5, Octn2
Uniprot ID	Q9Z0E8
免疫原	KLH偶联的小鼠Slc22a5合成肽
抗体亚型	IgG
纯化方式	亲和纯化
亚细胞定位	细胞膜

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应用

应用	物种	稀释	阳性样品
IHC/IF 免疫组织化学/免疫荧光	小鼠, 大鼠	1: 900-1: 1800	肾

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背景

Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. The encoded protein is a plasma integral membrane protein which functions both as an organic cation transporter and as a sodium-dependent high affinity carnitine transporter. The encoded protein is involved in the active cellular uptake of carnitine.

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图像

	IHC检测Solute carrier family 22 member 5蛋白(货号 GB112170). 样品: 小鼠肾, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: Tris-EDTA抗原修复液(pH 9.0) (G1203), 98℃, 20分钟. —抗: 1: 900稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.
	IHC检测Solute carrier family 22 member 5蛋白(货号 GB112170). 样品: 大鼠肾, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: Tris-EDTA抗原修复液(pH 9.0) (G1203), 98℃, 20分钟. —抗: 1: 900稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.