产品信息

蛋白质全称	白三烯B4受体1
别名	BLT, BLT1, BLTR, CMKRL1, G protein coupled receptor 16, GPR16, leukotriene B4 receptor, LTB4 R 1, LTB4 R1, LTB4R, LTB4R1, LTBR1, P2RY7, P2Y purinoceptor 7, P2Y7
Uniprot ID	O88855
免疫原	KLH偶联的小鼠Leukotriene B4 Receptor/BLT合成肽
抗体亚型	IgG
预测分子量./观测分子量.	38 kDa / 38 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	人	1: 500-1: 1000	293细胞, A549细胞, HaCaT细胞

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背景

Leukotriene B4 receptor 1 (LTB4R, also know as LTB4R1 or BLTR) is a G-protein coupled receptor for leukotriene B4, a potent chemoattractant that is primarily involved in inflammation, immune responses and host defence against infection . LTB4R is highly expressed in human leukocytes and is involved in the regulation of the inflammatory system.

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图像

WB检测Leukotriene B4 Receptor/BLT蛋白(货号 GB112910). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.