产品信息

蛋白质全称	胰岛素样因子3
别名	INSL3, insulin like 3 (Leydig cell), Ley-I-L, Leydig insulin like peptide, Relaxin like factor, RLF, RLNL, prepro-INSL3
Uniprot ID	O09107
免疫原	小鼠INSL3重组蛋白
抗体亚型	IgG
预测分子量./观测分子量.	14 kDa / 14 kDa
纯化方式	亲和纯化
亚细胞定位	分泌型

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠	1: 300-1: 800	睾丸
IHC/IF 免疫组织化学/免疫荧光	小鼠	1: 1500-1: 3000	睾丸

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背景

This gene encodes a member of the insulin-like hormone superfamily. The encoded protein is mainly produced in gonadal tissues. Studies of the mouse counterpart suggest that this gene may be involved in the development of urogenital tract and female fertility. This protein may also act as a hormone to regulate growth and differentiation of gubernaculum, and thus mediating intra-abdominal testicular descent. Mutations in this gene may lead to cryptorchidism. Alternate splicing results in multiple transcript variants.

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图像

	WB检测INSL3蛋白(货号 GB113558). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 800稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.
	IHC检测INSL3蛋白(货号 GB113558). 样品: 小鼠睾丸, 4%多聚甲醛 (货号G1101) 固定12-24小时. 抗原修复: 柠檬酸抗原修复液(干粉, pH 6.0) (G1201), 98℃, 20分钟. —抗: 1: 2000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 200稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.