产品信息

蛋白质全称	促肾上腺皮质激素释放因子受体 2
别名	Crhr2, CRHR2, CRF-RB, CRF2, CRFR2, HM-CRF, corticotropin releasing hormone receptor 2
Uniprot ID	Q60748
免疫原	KLH偶联的小鼠Corticotropin Releasing Factor Receptor 2合成肽
抗体亚型	IgG
预测分子量./观测分子量.	48 kDa / 48 kDa
纯化方式	亲和纯化

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应用

应用	物种	稀释	阳性样品
WB 免疫印迹	小鼠, 大鼠	1: 1000-1: 2000	心, 肌肉

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背景

The protein encoded by the CRHR2 gene belongs to the G-protein coupled receptor 2 family, and the subfamily of corticotropin releasing hormone receptor. This receptor shows high affinity for corticotropin releasing hormone (CRH), and also binds CRH-related peptides such as urocortin. CRH is synthesized in the hypothalamus, and plays an important role in coordinating the endocrine, autonomic, and behavioral responses to stress and immune challenge.

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图像

WB检测Corticotropin Releasing Factor Receptor 2蛋白(货号 GB11709). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 1000稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时.

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储存

储存条件	在-20℃下储存一年, 避免反复冻融.
储存缓冲液	含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液.

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注意:

1.本产品仅供研究使用.

2.建议用一抗稀释液(G2025)稀释本产品.