Anti-CCT2 Rabbit pAb
- 100 μL
产品信息
蛋白质全称 | T复合体蛋白1 β亚基 |
别名 | TCP-1-beta, CCT-beta, CCT2, CCTB, 99D8.1, Chaperonin containing t complex polypeptide 1 subunit 2, Chaperonin containing TCP1 subunit 2, MGC142074, MGC142076, MGC94480, Phosphocholine cytidylyltransferase 2, CTP, PRO1633, TCPB, Cct2, Cctb |
免疫原 | 小鼠CCT2重组蛋白 |
Uniprot ID | P80314 |
抗体亚型 | IgG |
纯化方式 | 亲和纯化 |
预测分子量. / 观测分子量. | 57 kDa / 57 kDa |
应用
| 应用 | 物种 | 稀释 | 阳性样品 |
| WB 免疫印迹 | 人, 小鼠, 大鼠 | 1: 500-1: 1000 | 肺, 肾, 脑 |
背景
TCP-1 beta is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). consists of 2 back to back rings, each containing eight different proteins. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha transducin.
图像
| WB检测CCT2蛋白(货号 GB11981). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. |
| WB检测CCT2蛋白(货号 GB11981). 样品: 经RIPA裂解液(货号G2002)处理的蛋白质. 封闭: 封闭: 3%脱脂牛奶(货号GC310001)溶于TBST溶液, 室温孵育1小时. —抗: 1: 500稀释, 4℃ 孵育过夜. 二抗: HRP标记山羊抗兔IgG (H+L) (货号GB23303), 1: 5000稀释, 室温孵育1小时. |
储存
| 储存条件 | 在-20℃下储存一年, 避免反复冻融. |
| 储存缓冲液 | 含0.15% ProClin300防腐剂, 100 μg/mL牛血清白蛋白和50%甘油的磷酸盐缓冲液. |
注意:
1.本产品仅供研究使用.
2.建议用一抗稀释液(G2025)稀释本产品.
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