Gene Name Synonym

Met, HGFR, C-Met, RCCP2, AUTS9, DFNB97

Species

Human

Accession

P08581

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Glu25-Thr932 is expressed with a 10×His tag at the C terminus

Molecular Weight

102.9 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a glycosylated receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin.

Data

SDS-PAGE

Bioactivity

Testing in progress

Recombinant Human Met Protein was determined by SDS-PAGE with Coomassie Blue.