DESCRIPTION Synonym VEGFA,VEGF alpha,RP1-261G23.1,MGC70609,MVCD1,VPF Species Rat A ccession P16612-2 Source CHO cells SPECIFICATIONS Amino acid sequence Met1-Arg190 is expressed with a 10×His tag at the C terminus Molecular Weight 23.8 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU·mg -1 Formulation Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND Vascular endothelial growth factor A (VEGFA),also known as Vascular permeability factor (VPF). VEGFA belongs to the PDGF/VEGF growth factor family. VEGFA is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGFA is produced by a group of three major isoforms as a result of alternative splicing and if any three isoforms are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in vessel defects and death of the full VEGFA knockout in mice. Data SDS-PAGE Bioactivity Active Recombinant Rat VEGF164 Protein was determined by SDS-PAGE with Coomassie Blue. 仅供科研用途，不可用于临床诊断！