DESCRIPTION Gene Name Synonym PDGF-DD, Platelet-derived growth factor D, SCDGFB Species Human Accession Q9GZP0 Source HEK293 cells SPECIFICATIONS Amino acid sequence Arg19-Arg370 is expressed with a 10×His tag at the C terminus Molecular Weight 40.7 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU mg -1 Formulation Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND PDGF-DD is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. PDGF-DD specifically binds to and activates its cognate receptor PDGFR-β, and participates in regulating cancer proliferation, transformation, and invasion through activating multiple downstream oncogenic pathways, resulting in tumor development and progression . Data SDS-PAGE Bioactivity Active Recombinant Human PDGFD Protein was determined by SDS-PAGE with Coomassie Blue. Measured in a cell proliferation assay using Balb/C 3T3 A31 mouse embryonic fibroblast cells. The ED50 for this effect is 1-2 μg/mL. 仅供科研用途，不可用于临床诊断！