DESCRIPTION Gene Name Synonym Brain-derived Neurotrophic Factor Species Mouse A ccession P21237 Source HEK293 cells SPECIFICATIONS Amino acid sequence Ala19-Arg249 is expressed with a 10×His tag at the C terminus Molecular Weight 27.5 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU·mg -1 Formulation Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND Brain-derived neurotrophic factor (BDNF) is a well-studied growth factor that serves many critical functions within the central nervous system (CNS). BDNF has a role in processes such as neuronal maturation, synapse formation and synaptic plasticity among others in the brain.BDNF is a member of the neurotrophin family that includes nerve growth factor (NGF), neurotrophin-3 (NT3), and neutorophin-4 (NT4). BDNF is widely expressed in the CNS and can exert profound effects on development, morphology, and synaptic plasticity and function in the brain. Data SDS-PAGE Bioactivity Recombinant Mouse BDNF Protein was determined by SDS-PAGE with Coomassie Blue. Measured in a cell proliferation assay using C6 cells. The ED50 for this effect is 0.1-1 µg/mL. 仅供科研用途，不可用于临床诊断！