DESCRIPTION Gene Name Synonym PDGF-C, Platelet derived growth factor C, VEGF-E, SCDGF Species Human Accession Q9NRA1 Source HEK293 cells SPECIFICATIONS Amino acid sequence Glu23-Gly345 is expressed with a 10×His tag at the C terminus Molecular Weight 36.8 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU mg -1 Formulation Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND PDGF-CC is a relatively new member of the PDGF family, abundantly expressed by different types of cells, such as tumor cells, endothelial cells (ECs), vascular smooth muscle cells (VSMCs), pericytes, fibroblasts, and macrophages. PDGF-CC binds to PDGFR-α and PDGFR-β, which are also expressed by many different cell types, including tumor cells, VSMCs, pericytes, and fibroblasts, all of which play important roles in angiogenesis. PDGF-CC is a potent angiogenic factor . Data SDS-PAGE Bioactivity Active Recombinant Human PDGFC Protein was determined by SDS-PAGE with Coomassie Blue. Measured in a cell proliferation assay using Balb/C 3T3 A31 mouse embryonic fibroblast cells. The ED50 for this effect is 5-10 μg/mL. 仅供科研用途，不可用于临床诊断！