DESCRIPTION Gene Name Synonym CSF2, GM-CSF, MGC131935 Species Human A ccession P04141 Source CHO cells SPECIFICATIONS Amino acid sequence Ala18-Glu144 is expressed with a 10×His tag at the N terminus Molecular Weight 15.9 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU·mg -1 Formulation Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND Colony-stimulating factor 2 (CSF-2) is a hematopoietic growth factor involved in the differentiation of various myeloid cells from hematopoietic progenitor cells. GM-CSF has been shown to play a crucial role in the pathogenesis of the experimental autoimmune encephalomyelitis (EAE), an animal model for multiple sclerosis. GM-CSF is a major cytokine produced by the encephalitogenic IL-17-producing T helper cells (Th17 cells) and required for Th17 cell-mediated EAE pathogenesis. GM-CSF stimulates the differentiation of hematopoietic progenitors to monocytes and neutrophils, and reduces the risk for febrile neutropenia in cancer patients. GM-CSF has been utilized in the clinical management of multiple disease processes. Most recently, GM-CSF has been incorporated into the treatment of malignancies as a sole therapy, as well as a vaccine adjuvant. Data SDS-PAGE Bioactivity Active Recombinant Human CSF2 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 25-30 kDa. CSF-2 stimulates the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.5 ng/ml. For Research Use Only! Ver. No.: V 1.0-202102 仅供科研用途，不可用于临床诊断！