DESCRIPTION Gene Name Synonym IFNB1, Interferon beta, IFN-beta, IFB, IFNB Species Mouse Accession P01575 Source HEK293 cells SPECIFICATIONS Amino acid sequence Ile22-Asn182 is expressed with a 10×His tag at the C terminus Molecular Weight 19.7 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU mg -1 Formulation Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND Human type I interferons (IFNs) are a large subgroup of interferon proteins that help regulate the activity of the immune system. Interferons bind to interferon receptors. All type I IFNs bind to a specific cell surface receptor complex known as the IFN-α receptor (IFNAR) that consists of IFNAR1 and IFNAR2 chains. The IFN-β proteins are produced in large quantities by fibroblasts. They have antiviral activity that is involved mainly in innate immune response. Two types of IFN-β have been described, IFN-β1 (IFNB1) and IFN-β3 (IFNB3) (a gene designated IFN-β2 is actually IL-6). IFN-β1 is used as a treatment for multiple sclerosis as it reduces the relapse rate. Furthermore, IFN-β1 can bind to a IFNAR1-IFNAR2 heterodimeric receptor, and can also function with IFNAR1 alone and independently of Jak-STAT pathways. Data SDS-PAGE Bioactivity Recombinant Mouse IFN-beta Protein was determined by SDS-PAGE with Coomassie Blue. Determined by its ability to induce STAT1/STAT2 activation in B16 F1 ISRE LUC reporter cells.The ED50 for this effect is 0.5-2 ng/mL. 仅供科研用途，不可用于临床诊断！