DESCRIPTION Gene Name Synonym IFN-gamma, IFNγ, Interferon-gamma, Interferon-γ Species Human A ccession P01579 Source HEK293 cells SPECIFICATIONS Amino acid sequence Gln24-Gly161 is expressed with a 10×His tag at the C terminus Molecular Weight 16.2 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU mg -1 Formulation Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND IFN gamma, also known as IFNG, is a secreted protein that belongs to the type II interferon family. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. Interferon (IFN)-γ is the uppermost cytokine implicated in anti-tumor immunity. With its cytostatic, pro-apoptotic and immune-provoking effects, IFN-γ plays a central role in the recognition and elimination of transformed cells. Interferon-γ (IFN-γ) also plays a key role in activation of cellular immunity and subsequently, stimulation of antitumor immune-response.IFN-γ is considered potentially useful for adjuvant immunotherapy for different types of cancer.IFN-γ is considered potentially useful for adjuvant immunotherapy for different types of cancer. The importance of IFN-γ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. Data SDS-PAGE Bioactivity Active Recombinant Human IFN-gamma Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 18-30 kDa. Determined by its ability to induce STAT1/STAT2 activation in Human COLO 205 ISRE LUC reporter cells.The ED50 for this effect is 1-5 ng/mL. 仅供科研用途，不可用于临床诊断！