DESCRIPTION Gene Name Synonym IL-9, Interleukin-9, IL9, Cytokine P40 Species Mouse A ccession P15247 Source HEK293 cells SPECIFICATIONS Amino acid sequence Gln19-Pro144 is expressed with a 10×His tag at the C terminus Molecular Weight 15.6 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU mg -1 Formulation Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND Interleukin 9, also known as IL-9, is a cytokine (cell signaling molecule) belonging to the group of interleukins. IL-9 is a multifunctional cytokine secreted by TH2 lymphocytes. Interleukin-9 is reported to play important roles in innate and adaptive immunity in extrarenal inflammatory diseases. The tolerogenic cytokine IL9 promotes T regulatory cell function and allergic airway inflammation. Eliminating endogenous IL9 enabled sensitization of host T cells to tumors, leading to their early rejection without the requirement of vaccines or immunomodulatory therapies. IL-9 predominantly produced by TH17 cells synergizes with TGF-β1 to differentiate naive CD4+ T cells into TH17 cells, while IL-9 secretion by TH17 cells is regulated by IL-23. IL-9 has been demonstrated to be involved in several diseases, such as cancer, autoimmunity and other pathogen-mediated immune-regulated diseases. Data SDS-PAGE Bioactivity Recombinant Mouse IL-9 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at30-40 kDa. Measured in a cell proliferation assay using MC/9 mouse mast cells. The ED50 for this effect is 1-5 ng/mL. For Research Use Only! Ver. No.: V 1.0-202102 仅供科研用途，不可用于临床诊断！