DESCRIPTION Gene Name Synonym CXCL8, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1 Species Human Accession P10145 Source HEK293 cells SPECIFICATIONS Amino acid sequence Met23-Ser99 is expressed with a 10×His tag at the C terminus Molecular Weight 10.3 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU mg -1 Formulation Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. There are many receptors on the surface membrane capable of binding IL-8, the most frequently studied types are the G protein-coupled serpentine receptors CXCR1 and CXCR2. Expression and affinity for IL-8 differs between the two receptors (CXCR1 > CXCR2). Data SDS-PAGE Bioactivity Recombinant Human IL-8 Protein was determined by SDS-PAGE with Coomassie Blue. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC-T1). The ED50 for this effect is typically 1-5 μg/mL. 仅供科研用途，不可用于临床诊断！