DESCRIPTION Gene Name Synonym IL-6, IL6, Interleukin-6, BSF2, HSF, IFNB2 Species Mouse A ccession P08505 Source HEK293 cells SPECIFICATIONS Amino acid sequence Phe25-Thr211 is expressed with a 10×His tag at the N terminus Molecular Weight 23.2 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU·mg -1 Formulation Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL6 is a potent inducer of the acute phase response. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule.IL-6 can lead to block the PI3K/AKT pathway. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. Data SDS-PAGE Bioactivity Active Recombinant Mouse IL-6 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 20-30 kDa. Measured in a cell proliferation assay using M-NFS-60 mouse B cell. The ED50 for this effects is 0.1-1 ng/mL. For Research Use Only! Ver. No.: V 1.0-202102 仅供科研用途，不可用于临床诊断！