DESCRIPTION G ene N ame Synonym IL-5, IL5, TRF, IL5, Interleukin-5 Species Mouse A ccession P04401 Source HEK293 cells SPECIFICATIONS Amino acid sequence Met21-Gly133 is expressed with a 10×His tag at the C terminus Molecular Weight 14.4 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU·mg -1 Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND IL-5 critically regulates expression of genes involved in cell survival, IgH switch recombination, maturation in B cells and genes required for growth, survival, and effector function of eosinophils. IL-5 was recognized as the major maturation and differentiation factor for eosinophils in mice and humans. IL-5 was originally defined as a T-cell-derived cytokine that triggers activated B cells for terminal differentiation into antibody-secreting plasma cells. IL-5 critically regulates expression of genes involved in proliferation, cell survival and maturation and effector functions of B cells and eosinophils. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. Interleukin 5 has long been associated with several allergic diseases, including allergic rhinitis and asthma. Data SDS-PAGE Bioactivity Active Recombinant Mouse IL-5 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 22-30 kDa. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 70 ng/mL. For Research Use Only! Ver. No.: V 1.0-202102 仅供科研用途，不可用于临床诊断！