DESCRIPTION Synonym IL-1 alpha, Il1a, Hematopoietin-1, LEM, LAF, Pro interleukin 1 alpha, IL1F1, Preinterleukin 1 alpha Species Human A ccession P01583 Source CHO cells SPECIFICATIONS Amino acid sequence Ser113-Ala271 is expressed with a 10×His tag at the C terminus Molecular Weight 19.4 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU·mg -1 Formulation Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND IL-1 alpha is a member of the interleukin 1 cytokine family. Located in the IL1 cluster, the IL1A gene is a product of IL1B gene duplication, and consequently IL-1α and IL-1β share a receptor, IL-1R1, and its downstream signaling. IL-1α has additional functions, represented by the presence of a nuclear localization signal within its pro-domain. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1α is a substrate of noncanonical inflammatory caspases. IL-1 alpha stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. Data SDS-PAGE Bioactivity Active Recombinant Human IL-1 alpha Protein was determined by SDS-PAGE with Coomassie Blue. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.2 μg/mL. For Research Use Only! Ver. No.: V 1.0-202102 仅供科研用途，不可用于临床诊断！