DESCRIPTION Gene Name Synonym Tnni3; Troponin I; cardiac muscle; Cardiac troponin I Species Rat A ccession P23693 Source HEK293 cells SPECIFICATIONS Amino acid sequence Ala2-Gly211 is expressed with a 10×His tag at the N terminus Molecular Weight 15.2 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU mg -1 Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND Tnni3 is the inhibitory subunit of the cardiac troponin (cTn) complex that is a sensor of the intracellular Ca2+ levels and modulates the interaction between the thick and thin filaments during muscle contraction. It mainly blocks the interaction between actin and myosin in the absence of Ca2+ and tightly regulated by phosphorylation. Data SDS-PAGE Bioactivity Recombinant Rat TNNI3 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 11-15 kDa. Immobilized Rat TNNI3, His Tag at 3 μg/mL (100 μL/well) can bind Anti-Cardiac Troponin I with a linear range of 0.009-0.078 μg/mL. （产品包装升级中，以实物为准。 ） 仅供科研用途，不可用于临床诊断！