DESCRIPTION Gene Name Synonym FN, FN1, FINC, CIG, ED-B, FINC, FNZ , GFND, GFND, LETS, MSF Species Mouse A ccession P11276 Source HEK293 cells SPECIFICATIONS Amino acid sequence Thr609-Ser2295 is expressed with a 10×His tag at the C terminus Molecular Weight 185.0 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU mg -1 Formulation Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND FN is a glycoprotein whose size ranges from 230 to 270 kDa and usually exists as a dimer, covalently linked by a pair of disulfide bonds at the C-termini. Fibronectin (FN) is a glycoprotein component of the extracellular matrix of the extracellular matrix (ECM) with roles in embryogenesis, development, and wound healing. FN is assembled by cells into viscoelastic fibrils that can bind upward of 40 distinct growth factors and cytokines. FN fibrils have unique mechanical properties, which allow them to alter mechanotransduction signals sensed and relayed by cells. More recently, FN has emerged as player in platelet thrombus formation and diseases associated with thrombosis including vascular remodeling, atherosclerosis, and cardiac repair following a myocardial infarct. Data SDS-PAGE Bioactivity Recombinant Mouse IFN-gamma Protein was determined by SDS-PAGE with Coomassie Blue. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human T lymphocyte cells.The ED50 for this effect is 10-20 μg/mL. For Research Use Only! Ver. No.: V 1.0-202102 （产品包装升级中，以实物为准。 ） 仅供科研用途，不可用于临床诊断！